Single-Cell Sequencing of Human Neural Organoids in a Model of Intracerebral Hemorrhage Reveals Temporally Dynamic Responses to Blood
Blood exposure induces transcriptomic changes in a temporal and cell type-specific manner, particularly mediated by astrocyte reactivity, angiogenesis, and impairment of neurogenesis. Early and complete removal of blood after 6 hours of blood exposure mitigates secondary neurotoxicity seen in 24-hour exposure to blood. Transcriptomic signatures of blood-mediated neurotoxicity may potentially be reversed by antiadrenergic, dopamine agonist, and vasodilatory mechanisms.
Authors
- James M. Vicari (ORCID: https://orcid.org/0000-0002-4633-9961)
- Braxton R. Schuldt (ORCID: https://orcid.org/0000-0002-1737-707X)
- Christopher P. Kellner (ORCID: https://orcid.org/0000-0003-4604-8205)
- Grace Rabinowitz (ORCID: https://orcid.org/0000-0002-0494-280X)
- Connie S. Lebakken (ORCID: https://orcid.org/0000-0002-5176-7370)
- Carina Seah (ORCID: https://orcid.org/0000-0003-1604-1838)
- William D. Richards
- Kaylie Greuel
- Kailyn T. Parham
Institutions
- University of Wisconsin System (US)
- Mount Sinai Health System (US)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Stroke
- Published
- 2025-09-04
- DOI
- https://doi.org/10.1161/strokeaha.125.051788
- Citations
- 1
- Primary Topic
- Immune cells in cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.80