Mycorrhization of Black Crowberry (Empetrum nigrum L.) After In Vitro Propagation with Mineral Fertilizers

Empetrun nigrum L. is a rare berry crop with a high content of biologically active substances, which are of great interest in medicine. In order to obtain sufficient transplants of this crop, in vitro propagation is preferred over other methods. It is known that a large percentage of propagated plants can be lost or damaged, not only at the stage of adaptation to non-sterile conditions, but also in the post-adaptation period. Plants may have weak growth due to poor root development and a lack of nutrients. Therefore, to improve the understanding of the plant requirements in the post-adaptation period of E. nigrum, clonal micropropagation was performed by combining mycorrhizal preparations containing (1) mycelium of Glomus sp., and humic substances, and (2) mycelium of Glomus sp., Trichoderma harzianum, and other mycorrhiza formation microorganisms with different types of mineral fertilizer (N20P20K20, N20P16K10(S5), and N19P9K10 + 2MgO). Analysis of the growth dynamics of ex vitro plants over 98 days of cultivation in containers under greenhouse conditions showed the effectiveness of mineral fertilizer and mycorrhizal preparations treatment. The total root length increased by 30–50% and the total shoot length by 40–80%. The survival was 95.8–100%.