Laser Powder Bed Fusion (LPBF) WE43 Magnesium Scaffold with EGCG/PDA-Functionalized Silk Fibroin Membrane for Antibacterial Osteoinductive Guided Bone Regeneration

Guided bone regeneration (GBR) is a prominent focus in biomedical materials research, yet few studies address practical clinical needs. GBR membranes must fulfill the "PASS" principles to be effective in surgery, but existing membranes often fall short in balancing antibacterial activity, controlled degradation, osteoinductive potential, and mechanical support. In this study, we employed laser powder bed fusion (LPBF) to fabricate a porous WE43 magnesium alloy scaffold suitable for large alveolar bone defects. We then used electrospinning and layer-by-layer (LBL) self-assembly to create a silk fibroin-based barrier membrane loaded with epigallocatechin gallate (EGCG) and polydopamine (PDA) for combined antibacterial and osteogenic functions. Laboratory tests confirmed that the WE43-F scaffolds and the EGCG/PDA-loaded membranes (SFE) are both biocompatible and bioresorbable. In vitro, they enhanced new bone formation by upregulating alkaline phosphatase (ALP) and RUNX2 gene expression while effectively inhibiting <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. In a rat maxillary defect model, the WE43-F/SFE composite system demonstrated superior bone regeneration, yielding the largest area of new bone and the most mature collagen-bone matrix, with a bone volume to total volume ratio (BV/TV) of 28.06%. In this study, we assembled WE43-F scaffolds with PDA-EGCG electrospun membranes to create a biodegradable GBR system that delivers synergistic antibacterial and osteogenic effects. Its antimicrobial efficacy and bone-regenerative capacity were confirmed through in vitro assays, cell culture experiments, antibacterial testing, and a rat mandibular defect model.