Clean up of Index PCRs using SPRI Beads v1
This protocol describes the cleanup of index PCR products using SPRI bead-based size selection at a 1.4:1 bead-to-DNA ratio. The process includes bead binding, ethanol washes, DNA elution, and quantification using Qubit or other methods. The expected result is purified DNA libraries with concentration measurements for downstream analysis.
Authors
- Deon de Jager (ORCID: https://orcid.org/0000-0003-4388-2624)
- Eline D. Lorenzen (ORCID: https://orcid.org/0000-0002-6353-2819)
- Michael V. Westbury (ORCID: https://orcid.org/0000-0003-0478-3930)
- Vanssy Li
- Alba Ray-Iglesia
Institutions
- University of Copenhagen (DK)
- IT University of Copenhagen (DK)
Publication Details
- Published
- 2025-05-08
- DOI
- https://doi.org/10.17504/protocols.io.36wgqqkdxgk5/v1
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00