Electrostatically Adjustable AuNR-Mediated Thermal-Start Isothermal Nucleic Acid Amplification

Loop-mediated isothermal amplification (LAMP) holds great promise for rapid nucleic acid detection. However, its application is hindered by the notable occurrence of false positives. In this work, gold nanorod (AuNR)-mediated hot start effect, which adsorbs primers and controls the release of single-stranded DNA (ssDNA) primers at about 50 °C, was demonstrated. The AuNR-mediated LAMP reaction for detecting African horse sickness virus (AHSV) showed high selectivity against other DNA fragments, even at high magnesium concentrations. Furthermore, the assay achieves excellent sensitivity with a detection limit of 100 copies/μL. Additionally, the method displays good repeatability from 50 to 65 °C (optimal), and the coefficient of variation of Tt at the same concentration of nucleic acids is less than 5%. Our study optimizes the LAMP reaction using AuNRs, thereby reducing false positives and offering great potential for clinical diagnosis.

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Publication Details

Journal
ACS Omega
Published
2025-09-04
DOI
https://doi.org/10.1021/acsomega.5c05097
Citations
2
Primary Topic
Plant Virus Research Studies
Type
article
Field-Weighted Citation Impact
3.50

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article

Electrostatically Adjustable AuNR-Mediated Thermal-Start Isothermal Nucleic Acid Amplification

Jilie Kong, Xueen Fang, Jiale Gao, Huiwen Xiong et al.
2 citations
ACS Omega
Plant Virus Research Studies
3.50
article

Electrostatically Adjustable AuNR-Mediated Thermal-Start Isothermal Nucleic Acid Amplification

Jilie Kong, Xueen Fang, Jiale Gao, Huiwen Xiong, Hanhua Li, Lin Liu, Qin Chen, Wenhao Weng, Dilimulati Yishake
article en
2 citations

Abstract

Loop-mediated isothermal amplification (LAMP) holds great promise for rapid nucleic acid detection. However, its application is hindered by the notable occurrence of false positives. In this work, gold nanorod (AuNR)-mediated hot start effect, which adsorbs primers and controls the release of single-stranded DNA (ssDNA) primers at about 50 °C, was demonstrated. The AuNR-mediated LAMP reaction for detecting African horse sickness virus (AHSV) showed high selectivity against other DNA fragments, even at high magnesium concentrations. Furthermore, the assay achieves excellent sensitivity with a detection limit of 100 copies/μL. Additionally, the method displays good repeatability from 50 to 65 °C (optimal), and the coefficient of variation of Tt at the same concentration of nucleic acids is less than 5%. Our study optimizes the LAMP reaction using AuNRs, thereby reducing false positives and offering great potential for clinical diagnosis.

ACS OmegaVol. 10(36)
Shanghai University (CN), Tongji University (CN), Shanghai Jiao Tong University (CN), Fudan University (CN), Shanghai Children's Hospital (CN), Institute of Infection and Immunity (CA)
Natural Science Foundation of Shanghai, National Natural Science Foundation of China, Science and Technology Commission of Shanghai Municipality, Shanghai Municipal Commission of Agriculture and Rural Affairs, National Ten Thousand Talent Program
Openalex Percentile: Top 7%
Plant Virus Research Studies
3.50
DOIOpen Access PDFOpenAlex
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