Purification of in vitro transcribed RNA with the Zymo Research RNA Clean & Concentrator™-25 Kit v1

This is a protocol for purification of in vitro transcribed RNA via the Zymo Research RNA Clean & Concentrator™-25 kit. It works with capped and uncapped mRNA (usually >600 nt) with or without poly(A)-tails , shRNA (49 nt) and single guide sgRNA (100 nt) and yields RNA of high purity and concentration.

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Publication Details

Published
2025-06-15
DOI
https://doi.org/10.17504/protocols.io.yxmvmm8y5v3p/v1
Primary Topic
Molecular Biology Techniques and Applications
Type
article
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article

Purification of in vitro transcribed RNA with the Zymo Research RNA Clean & Concentrator™-25 Kit v1

Christian CR Renicke
Molecular Biology Techniques and Applications
article

Purification of in vitro transcribed RNA with the Zymo Research RNA Clean & Concentrator™-25 Kit v1

Christian CR Renicke
article en

Abstract

This is a protocol for purification of in vitro transcribed RNA via the Zymo Research RNA Clean & Concentrator™-25 kit. It works with capped and uncapped mRNA (usually >600 nt) with or without poly(A)-tails , shRNA (49 nt) and single guide sgRNA (100 nt) and yields RNA of high purity and concentration.

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Molecular Biology Techniques and Applications
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