Purification of in vitro transcribed RNA with the Zymo Research Quick-RNA Miniprep Kit v1

This is a protocol for purification of in vitro transcribed RNA via the Zymo Research Quick-RNA Miniprep Kit. It works with capped and uncapped mRNA (usually >600 nt) with or without poly(A)-tails , shRNA (49 nt) and single guide sgRNA (100 nt) and yields RNA of high purity and concentration.

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Publication Details

Published
2025-06-16
DOI
https://doi.org/10.17504/protocols.io.3byl41prolo5/v1
Primary Topic
Molecular Biology Techniques and Applications
Type
article
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article

Purification of in vitro transcribed RNA with the Zymo Research Quick-RNA Miniprep Kit v1

Christian CR Renicke
Molecular Biology Techniques and Applications
article

Purification of in vitro transcribed RNA with the Zymo Research Quick-RNA Miniprep Kit v1

Christian CR Renicke
article en

Abstract

This is a protocol for purification of in vitro transcribed RNA via the Zymo Research Quick-RNA Miniprep Kit. It works with capped and uncapped mRNA (usually >600 nt) with or without poly(A)-tails , shRNA (49 nt) and single guide sgRNA (100 nt) and yields RNA of high purity and concentration.

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Molecular Biology Techniques and Applications
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Purification of in vitro transcribed RNA with the Zymo Research Quick-RNA Miniprep Kit v1 — Christian CR Renicke · (2025) | TGRS Research Map | TGRS