Electroporation of Aiptasia zygotes v1
This is a step-by-step guide to electroporation of Aiptasia (Exaiptasia diaphana) zygotes with different genetic constructs (plasmid DNA, mRNA, shRNA). It allows transformation of hundreds to thousands of zygotes in one session for anyone, who can hold and operate a micropipette. Larvae develop normally and can be used for experimentation, including establishment of symbiosis with compatible algal strains.
Authors
- Christian CR Renicke (ORCID: https://orcid.org/0000-0003-3226-9840)
- Lorraine Ling (ORCID: https://orcid.org/0000-0003-0233-1543)
Publication Details
- Published
- 2025-05-24
- DOI
- https://doi.org/10.17504/protocols.io.rm7vzqdwrvx1/v1
- Primary Topic
- Microbial Inactivation Methods
- Type
- article
- Field-Weighted Citation Impact
- 0.00