Production and purification of plasmid DNA with the Zymo Research ZymoPURE™ II Plasmid Maxiprep kit v1

This protocol is for production and purification of high yields of highly pure and endotoxin-free plasmid DNA with the Zymo Research ZymoPURE™ II Plasmid Maxiprep Kit. The total yield can be up to 3 mg of DNA, we typically achieve between 500 and 1000 µg in ≥300 µL elution volume. The concentration and purity is sufficient for electroporation in Aiptasia zygotes.