Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization

Respiratory tract infections pose considerable global health challenges. Upper airway colonization is pivotal to these infections, including those caused by <i>Bordetella</i> species. We identified an oligosaccharide, bordetellae colonization oligosaccharide (b-Cool), crucial for early nasal colonization of <i>Bordetella bronchiseptica</i>. We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including <i>Bordetella pertussis</i>, which causes whooping cough in humans. A <i>B. bronchiseptica</i> mutant lacking b-Cool (Δ<i>b-Cool</i>) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of Δ<i>b-Cool</i> was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.

Authors

Institutions

Publication Details

Journal
Science Advances
Published
2025-09-03
DOI
https://doi.org/10.1126/sciadv.adw7764
Primary Topic
Bacterial Infections and Vaccines
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization

Evgeny Vinogradov, Junfei Zhu, John Glushka, Jillian Masters et al.
Science Advances
Bacterial Infections and Vaccines
article

Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization

Evgeny Vinogradov, Junfei Zhu, John Glushka, Jillian Masters, Christopher M. Evans, Rhoads Stevens, Kalyan K. Dewan, Amanda D. Caulfield, Colleen Sedney, Yang Su, M Vu, Andrew Preston, Thomas M. Krunkosky, A. Aldrin Joshua, Maiya Callender, Maor Bar‐Peled, Eric T. Harvill, Emily Gaskill, Dylan Woolsey
article en

Abstract

Respiratory tract infections pose considerable global health challenges. Upper airway colonization is pivotal to these infections, including those caused by <i>Bordetella</i> species. We identified an oligosaccharide, bordetellae colonization oligosaccharide (b-Cool), crucial for early nasal colonization of <i>Bordetella bronchiseptica</i>. We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including <i>Bordetella pertussis</i>, which causes whooping cough in humans. A <i>B. bronchiseptica</i> mutant lacking b-Cool (Δ<i>b-Cool</i>) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of Δ<i>b-Cool</i> was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.

Science AdvancesVol. 11(36)
University of Georgia (US), Rocky Mountain MS Center (US), National Research Council Canada (CA), University of Bath (GB), University of Colorado Anschutz Medical Campus (US)
Openalex Percentile: Top 78%
Bacterial Infections and Vaccines
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.