Genome-Wide Identification and Expressional Analysis of the TIFY Gene Family in Eucalyptus grandis

The <i>TIFY</i> gene family participates in crucial processes including plant development, stress adaptation, and hormonal signaling cascades. While the <i>TIFY</i> gene family has been extensively characterized in model plant systems and agricultural crops, its functional role in <i>Eucalyptus grandis</i>, a commercially valuable tree species of significant ecological and economic importance, remains largely unexplored. In the present investigation, systematic identification and characterization of the <i>TIFY</i> gene family were performed in <i>E. grandis</i> using a combination of genome-wide bioinformatics approaches and RNA-seq-based expression profiling. Nineteen <i>EgTIFY</i> genes were identified in total and further grouped into four distinct subfamilies, TIFY, JAZ (subdivided into JAZ I and JAZ II), PPD, and ZML, based on phylogenetic relationships. These genes exhibited considerable variation in gene structure, chromosomal localization, and evolutionary divergence. Promoter analysis identified a multitude of cis-acting motifs involved in mediating hormone responsiveness and regulating abiotic stress responses. Transcriptomic profiling indicated that <i>EgJAZ9</i> was strongly upregulated under methyl jasmonate (JA) treatment, suggesting its involvement in JA signaling pathways. Taken together, these results offer valuable perspectives on the evolutionary traits and putative functional roles of <i>EgTIFY</i> genes.