Evaluation of Effects of Er,Cr:YSGG Laser, Eggshell Solution, and Their Combination on the Remineralization of Erosive Lesion Caused by Lemonade

<b>Introduction:</b> Erosion is the loss of minerals by acids without the intervention of microorganisms. Complications caused by erosion make the use of therapeutic agents necessary. This study aims to survey the effects of the eggshell, the Er,Cr:YSGG laser, and their combination on the remineralization of erosive lesions caused by lemonade. <b>Methods:</b> The microhardness of 40 impacted third molar teeth was measured in this laboratory study. Then, the samples were subjected to an erosion challenge using a regular lemonade drink. After measuring microhardness, the samples were exposed to remineralizing materials: Er, Cr:YSG laser, eggshell, laser Er,Cr:YSGG, eggshell, and fluoride (positive control), and artificial saliva (negative control). Finally, data were analyzed via Kolmogorov-Smirnov, mixed-model analysis of variance (ANOVA), and LSD post hoc tests. <b>Results:</b> The microhardness of permanent third molar teeth, which was reduced after using lemonade, increased significantly after laser application compared to fluoride and artificial saliva. No significant difference was observed between the five experimental groups while there were statistically significant differences between the three stages of the study. Moreover, there was a significant interaction between the groups and stages (group * stage). The SMH of fluoride, artificial saliva, and laser+solution groups did not significantly change in the three stages of the study whilst the SMH of laser and solution groups significantly changed in the three stages. The SMH of the laser group had the most increasing trend (between erosive and treatment stages), followed by laser+solution and solution groups. However, the change in the laser+solution group was not statistically noticeable. <b>Conclusion:</b> Based on the results of this study, the Er,Cr:YSGG laser alone can be effective in the remineralization of erosive lesions caused by lemonade.