Effects of the in vivo treatment of radioactive implant-induced skin wounds with adipose-derived mesenchymal stem cells (ASCs)

Introduction. Adipose tissue-derived mesenchymal stem cells (ASCs) play a crucial role in healing skin wounds caused by radiation. Herein is demonstrated a patented, novel radioactive implant designed for research on the effects of radiation and the wound repair process. Material and methods. Inbred LEW/W rats were used as both ASCs donors and recipients of radiation. A radiation wound was induced by inserting a I125 implant under the dorsal skin. After irradiating the skin with doses of 10 Gy over 3 days or 18 Gy over 5.5 days, ASCs were injected into the wound area at weekly intervals. Results. Treatment with ASCs altered the immune system’s response to the radiation wound, promoted neoangiogenesis and reduced necrotic areas at the wound site. Conclusions. The present in vivo irradiation technique may enhance the understanding and refinement of brachytherapy techniques, utilizing tissue-implanted radiation sources with the ability to shield adjacent tissues effectively.