<i>Adenylate kinase</i> gene polymerase chain reaction-restriction fragment length polymorphism to differentiate <i>Escherichia coli</i>, <i>Escherichia fergusonii</i>, and <i>Citrobacter freundii</i>:
This molecular technique provides a rapid and accurate method to discriminate between closely related bacterial species and is promising for clinical diagnostics and epidemiological studies. Thus, our adk PCR/restriction digestion assay is a valuable tool for the advancement of bacterial typing methods for EC, EF, and CF, and contributes to the ongoing exploration of microbial diversity and epidemiology.
Authors
- Bokyung Kim (ORCID: https://orcid.org/0009-0002-8718-1184)
- Md Shohel Rana (ORCID: https://orcid.org/0009-0003-3268-7273)
- Yoon-Jung Choi (ORCID: https://orcid.org/0000-0002-0623-3185)
- Shukho Kim (ORCID: https://orcid.org/0000-0003-3322-7984)
- Ram Hari Dahal (ORCID: https://orcid.org/0000-0001-5887-6898)
- Jungmin Kim (ORCID: https://orcid.org/0000-0002-1795-1844)
- John Shin
Institutions
- Kyungpook National University (KR)
Publication Details
- Journal
- Journal of Yeungnam Medical Science
- Published
- 2025-09-01
- DOI
- https://doi.org/10.12701/jyms.2025.42.52
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea