Absorption, Thermal Relaxation Time, and Beam Penetration Depth of Laser Wavelengths in Ex Vivo Porcine Gingival Tissues

<b>Background/Objectives</b>: The laser beam absorption and thermal relaxation time (TRT) in oral tissues are key to optimizing treatment parameters. The aim of this study is to (1) evaluate, in an ex vivo study, the percentage of attenuation and transmittance of each wavelength as a function of tissue thickness; (2) determine the global absorption coefficient, α, of pig gingival tissue for the most commonly used wavelengths in dentistry; (3) calculate the thermal relaxation time (TRT) of oral tissue for these wavelengths; and (4) determine their corresponding penetration depths. <b>Methods:</b> We measured the transmission of different laser wavelengths through pig oral gingival tissues (Mandibular labial gingiva). We placed each tissue sample between two glass slides with minimal light attenuation. The input and output powers were measured after irradiating the tissue at different specific wavelengths: 450 nm, 480 nm, 532 nm, 632 nm, 810 nm, 940 and 980 nm, 1064 nm, 1341, 2780 nm and 2940 nm. After calculating the transmittance values, we plotted transmittance curves for each wavelength. Using the Beer-Lambert law, we then calculated the absorption coefficient (α) of each wavelength in the oral gingival tissue. Absorption coefficients were then used to calculate the TRT and penetration depth for each wavelength. <b>Results:</b> Among the tested wavelengths, 810 nm exhibited the lowest absorption in ex vivo porcine gingival tissue (α = 9.60 cm^-1). The 450 nm blue laser showed moderate absorption (α = 26.8 cm^-1), while the Er:YAG laser at 2940 nm demonstrated the highest absorption (α = 144.8 cm^-1). We ranked the wavelengths from most absorbed to least absorbed by porcine oral gingival mucosa as follows: 2940 nm > 2780 nm > 450 nm > 480 nm > 532 nm > 1341 nm > 632 nm > 940 nm > 980 nm > 1064 nm > 810 nm. <b>Conclusions:</b> Absorption and the TRT vary significantly across wavelengths. Erbium lasers are characterized by the highest absorption and minimal light penetration. Infrared diodes, particularly the 810 nm wavelength, showed the lowest absorption and deepest tissue penetration and exhibited the highest thermal relaxation time. The 480 nm laser demonstrated greater absorption by porcine gingival tissue compared to the 532 nm laser. These findings provide evidence-based guidance for wavelength selection in dental treatments and photobiomodulation, enabling improved precision, safety, and therapeutic efficacy in clinical practice.