High-quality genome assembly and annotation of live animal vaccine bacteria strains in South Korea

Selecting and managing suitable seed strains according to standard principles is a critical factor in manufacturing high-quality biological products. In this study, we determined the complete genomes of live animal vaccine bacterial strains used in South Korea and comprehensively analyzed their gene annotations. This detailed genomic analysis provides a robust foundation for identifying and verifying whether manufacturers utilize the same seed strains at the strain level. Furthermore, these data serve as a valuable reference to ensure consistent mass production and reliable quality control of biological products. Complete genome sequencing and annotation were performed on five bacterial strains (Erysipelothrix rhusiopathiae NL-11, Clostridium chauvoei B, Bordetella bronchiseptica 12-147-AFQ2, Bordetella bronchiseptica S-55, and Mycoplasmoides gallisepticum str. F) obtained directly from live attenuated vaccines. A hybrid genome assembly approach combining Illumina short reads (NovaSeq 6000) and Oxford Nanopore long reads (MinION Mk1B) was used to obtain complete, high-quality, gap-free, and well-annotated genome sequences. The genome sizes of E. rhusiopathiae NL-11, C. chauvoei B, B. bronchiseptica 12-147-AFQ2, B. bronchiseptica S-55, and M. gallisepticum str. F were 1.78, 2.40, 5.34, 5.34, and 0.95 Mb, respectively.