Modulation of NK Cell Properties by ESKAPE Group Bacteria
Functions of natural killer cells (NK cells) can be modulated by environmental stimuli. However, the role of bacterial components in this modulation remains an area of ongoing research. This study investigates how bacterial supernatants influence NK cell function-including cytotoxicity, phenotype, and cytokine and mRNA production-following co-culture. These parameters were measured by flow cytometry and RT-PCR. We found that NK cells express TLR2 and TLR5, and that exposure to supernatants from ESKAPE group bacteria modified their cytotoxicity against JEG-3 and K-562 cell lines and their <i>NKG2A</i> and <i>IL-10</i> mRNA levels. Our findings indicate that bacteria can modify NK cell features and their interactions with other cells in the microenvironment.
Authors
- Д. И. Соколов (ORCID: https://orcid.org/0000-0002-5749-2531)
- Sergey Orlov (ORCID: https://orcid.org/0000-0002-3134-1989)
- Арег А. Тотолян (ORCID: https://orcid.org/0000-0003-4571-8799)
- Л. А. Краева (ORCID: https://orcid.org/0000-0002-9115-3250)
- Elizaveta Tyshchuk (ORCID: https://orcid.org/0000-0001-6051-9048)
- E. A. Denisova
- Polina Grebenkina (ORCID: https://orcid.org/0000-0002-5229-9732)
- Zoia R. Korobova (ORCID: https://orcid.org/0000-0003-0535-5014)
- Varvara Juchina
- Ananstasia Gulina
Institutions
- First Pavlov State Medical University of St. Petersburg (RU)
- Research Institute of Obstetrics and Gynecology named after D.O. Ott (RU)
- Saint Petersburg Pasteur Institute (RU)
- Institute of Experimental Medicine (RU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2025-08-30
- DOI
- https://doi.org/10.3390/ijms26178449
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Russian Science Foundation