Structure-Guided Stapling of Dimeric Conformations and Linker Engineering Enhance Thermostability and Fine-Tune Activity of Bispecific VHH Cytokine Agonists
Our findings have important implications for the design and engineering of bispecific VHHs or sdAbs, offering a novel strategy for tuning their activity and increasing their stability.
Authors
- Sandro Vivona (ORCID: https://orcid.org/0000-0003-2340-3219)
- Deepti Rokkam
- Raphael Trenker (ORCID: https://orcid.org/0000-0003-1748-0517)
- Ivan Cheng (ORCID: https://orcid.org/0000-0003-0357-8441)
- Martin Oft (ORCID: https://orcid.org/0000-0001-9833-2326)
- René de Waal Malefyt (ORCID: https://orcid.org/0000-0002-2663-383X)
- Patrick J. Lupardus (ORCID: https://orcid.org/0000-0002-8662-074X)
- Verenice Paredes (ORCID: https://orcid.org/0000-0002-0267-0230)
- Priyanka Balasubrahmanyam
- Andrew Morin
Institutions
- Synthego (United States) (US)
Publication Details
- Journal
- Antibodies
- Published
- 2025-09-01
- DOI
- https://doi.org/10.3390/antib14030074
- Citations
- 1
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.80