In Vitro Study of the Effect of 1.0% Sodium Hyaluronate on Bacterial Strains and Antibiotics

During anterior segment eye surgeries, ophthalmic viscosurgical devices (OVDs), primarily containing hyaluronic acid, protect the corneal endothelium and help maintain the anterior chamber with reduced trauma. We aimed to investigate the interaction of sodium hyaluronate 1.0% with Gram-positive and Gram-negative bacteria, and its interactions with prophylactic intracameral antibiotics moxifloxacin and cefuroxime. Four quality control bacterial strains were used in vitro to conduct five experiments using either cefuroxime or moxifloxacin. Our experiment included five stages: 1. Testing the ability of OVD to retain bacteria before antibiotic exposure. 2. Examining antibiotic-bacteria interactions in the presence of OVD. 3. Simulating aqueous humor circulation. 4. Evaluating the effect of OVD residuals on antibiotic retention. 5. Analyzing the interaction of bacteria and antibiotics within the OVD. Results showed a significant decrease in bacterial counts (p