Chromosome-level genome assembly of the Tyrrhenian tree frog (Hyla sarda)

The Tyrrhenian tree frog (Hyla sarda) is a small cryptically coloured amphibian found in Corsica, Sardinia, and the Tuscan Archipelago. Investigation into the species' evolutionary history has revealed phenotypic changes triggered by glaciation-induced range expansion, but understanding the genetic basis of this trait variation has been hampered by the lack of a reference genome. To address this, we assembled a chromosome-level genome of Hyla sarda using PacBio HiFi long reads, Bionano optical maps, and Hi-C data. The assembly comprises 13 assembled chromosomes, spanning a total length of 4.15 Gb with a scaffold N50 of 385 Mb, a BUSCO completeness of 94.60%, and a k-mer completeness of 98.30%. Approximately 75% of the genome consists of repetitive elements. We annotated 22,847 protein-coding genes with a BUSCO completeness of 94.60% and an OMArk completeness of 93.74%. This high-quality assembly provides a valuable resource for studying phenotypic evolution and its genomic basis during range expansion, and will assist future investigations into the population and conservation genomics of Hyla sarda.

Authors

Institutions

Publication Details

Journal
Scientific Data
Published
2025-09-02
DOI
https://doi.org/10.1038/s41597-025-05760-9
Primary Topic
Genomics and Phylogenetic Studies
Type
article
Field-Weighted Citation Impact
0.00

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Chromosome-level genome assembly of the Tyrrhenian tree frog (Hyla sarda)

Brian I. O’Toole, Roberta Bisconti, Giulio Formenti, Daniele Salvi et al.
Scientific Data
Genomics and Phylogenetic Studies
article

Chromosome-level genome assembly of the Tyrrhenian tree frog (Hyla sarda)

Brian I. O’Toole, Roberta Bisconti, Giulio Formenti, Daniele Salvi, Erich D. Jarvis, Nivesh Jain, Andrea Chiocchio, Jennifer Balacco, Linelle Ann L. Abueg, Josephine R. Paris, Daniele Canestrelli, Dominic Absolon, Tatiana Tilley
article en

Abstract

The Tyrrhenian tree frog (Hyla sarda) is a small cryptically coloured amphibian found in Corsica, Sardinia, and the Tuscan Archipelago. Investigation into the species' evolutionary history has revealed phenotypic changes triggered by glaciation-induced range expansion, but understanding the genetic basis of this trait variation has been hampered by the lack of a reference genome. To address this, we assembled a chromosome-level genome of Hyla sarda using PacBio HiFi long reads, Bionano optical maps, and Hi-C data. The assembly comprises 13 assembled chromosomes, spanning a total length of 4.15 Gb with a scaffold N50 of 385 Mb, a BUSCO completeness of 94.60%, and a k-mer completeness of 98.30%. Approximately 75% of the genome consists of repetitive elements. We annotated 22,847 protein-coding genes with a BUSCO completeness of 94.60% and an OMArk completeness of 93.74%. This high-quality assembly provides a valuable resource for studying phenotypic evolution and its genomic basis during range expansion, and will assist future investigations into the population and conservation genomics of Hyla sarda.

Scientific DataVol. 12(1)
Marche Polytechnic University (IT), Università degli Studi della Tuscia (IT), University of L'Aquila (IT), Wellcome Sanger Institute (GB), Rockefeller University (US)
Howard Hughes Medical Institute, Ministero dell’Istruzione, dell’Università e della Ricerca
Openalex Percentile: Top 80%
Genomics and Phylogenetic Studies
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.