Figure 3 from Dietary Fiber Lacks a Consistent Effect on Immune Checkpoint Blockade Efficacy Across Diverse Murine Tumor Models

<p>Grain-based chow favors ICB efficacy in MC-38 allografts, independently of dietary fiber. <b>A,</b> Study design for transplantable tumor models shown in <a href="#fig3" target="_blank">Fig. 3</a> and <a href="#fig4" target="_blank">4</a>. The first two MC-38 experiments (<b>B–E</b>) did not include a purified high-fiber dietary arm. Chow is intrinsically high fiber. <b>B</b> and <b>C,</b> Mean tumor volumes and fraction of mice free of tumors larger than 700 mm<sup>3</sup> in mice implanted with subcutaneous MC-38 tumors, fed with chow or a low–fiber-purified diet, and treated with anti–PD-1 or isotype control in a study performed in CRL facilities. <i>n</i> = 8 for isotype control and <i>n</i> = 12 for anti–PD-1 groups. <b>D</b> and <b>E,</b> Same as in <b>B</b> and <b>C</b> but in a study performed in PU facilities. <i>n</i> = 10. <b>F</b> and <b>G,</b> Same as in <b>B</b> and <b>C</b> for mice fed with chow or purified diets with low or high insoluble fiber (cellulose, LC, or HC, respectively) and low or high soluble fiber (inulin). <i>n</i> = 10. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001, two-way ANOVA with the Tukey <i>post hoc</i> test at endpoint (day 38 for <b>F</b>) for <b>B</b>, <b>D</b>,<b> </b>and<b> F </b>and<b></b>log-rank test for <b>C</b>, <b>E</b>, and <b>G</b>. Values are mean ± SEM. (<b>A,</b> Created with <a href="http://sciencefigures.org" target="_blank">sciencefigures.org</a>.)</p>