In <i>Saccharomyces cerevisiae</i>, the molecular chaperone proteins Ssb1 and Ssb2 upregulate ABC transporter genes, and their upregulation may play a role in the release of quorum-sensing molecules that induce cell growth arrest during t

In <i>Saccharomyces cerevisiae</i>, the molecular chaperone proteins Ssb1 and Ssb2 (Ssb1/2) and the cochaperone ribosome-associated complex (Zuo1 and Ssz1) localize around the ribosome tunnel exit, assisting in the maturation of nascent polypeptides. Exogenous expression of the Zuo1 C-terminus or the Ssz1 N-terminus-but not Ssb1/2-independently activates the transcription factor Pdr1 (but not Pdr3), enhances the transcription of the ATP-binding cassette (ABC) transporter genes <i>PDR5</i>, <i>SNQ2</i>, and <i>YOR1</i>, and increases pleiotropic drug resistance. Furthermore, upregulation of ABC transporter genes by <i>ZUO1</i> and <i>SSZ1</i> leads to the release of quorum-sensing molecules, which cause cell growth arrest during diauxic shifts. In this study, we examined whether <i>SSB1/2</i> are required for the expression of ABC transporter genes and the release of quorum-sensing molecules that lead to cell growth arrest during diauxic shifts. Our results show that Ssb1/2 increased the mRNA levels of <i>PDR5</i>, <i>SNQ2</i>, and <i>YOR1</i> during the late logarithmic growth phase and increased resistance to cycloheximide and fluconazole, possibly via the same pathway as Zuo1 or Ssz1. Furthermore, Ssb1/2 induced <i>PDR5</i> expression and resistance to cycloheximide and fluconazole, possibly via the same pathway as Pdr3 (but not Pdr1). In addition, it was suggested that Ssb1/2 are involved in the release of quorum-sensing molecules into the culture medium, which could signal cell growth arrest during diauxic shifts. This work provides useful knowledge regarding genetic interactions between the ribosome-associated molecular chaperone and cell growth arrest during diauxic shifts.