Figure S1 from CRISPR Screens Identify POLB as a Synthetic Lethal Enhancer of PARP Inhibition Exclusively in BRCA-Mutated Tumors
<p>Supplementary Figure S1. MAGeCK analysis of CRISPR screens – related to Figure 1. A) Volcano plots showing MAGeCK analysis of CRISPR-Cas9 screens performed in BRCA1 wildtype cancer cell lines, A549 (left) and CAL27 (right). Genes on the left are depleted and genes on the right are enriched in olaparib treatment compared to DMSO treatment. B) Volcano plots showing MAGeCK analysis of CRISPR-Cas9 screens performed in DLD-1 WT (left) and DLD-1 BRCA2 het (right) cells. Genes on the left are depleted and genes on the right are enriched in olaparib treatment compared to DMSO treatment. C) Volcano plots showing MAGeCK analysis of CRISPR-Cas9 screens performed in DLD-1 BRCA2 mutant (left) and DLD-1 BRCA2 null (right) cells. Genes on the left are depleted and genes on the right are enriched in olaparib treatment compared to DMSO treatment.</p>
Authors
- Brian Doyon
- Madhavi Bandi (ORCID: https://orcid.org/0000-0002-7562-0170)
- Tianshu Feng (ORCID: https://orcid.org/0000-0002-9445-4876)
- William D. Mallender
- Robert Tjin Tham Sjin (ORCID: https://orcid.org/0000-0002-3809-8424)
- Jannik N. Andersen (ORCID: https://orcid.org/0000-0003-4528-2120)
- Erik Wilker (ORCID: https://orcid.org/0000-0003-4535-178X)
- Douglas A. Whittington (ORCID: https://orcid.org/0000-0002-5946-8543)
- Alan Huang (ORCID: https://orcid.org/0000-0001-5501-0212)
- Yi Yu (ORCID: https://orcid.org/0000-0002-2223-337X)
- Xuewen Pan (ORCID: https://orcid.org/0000-0002-0148-5631)
- Katherine Lazarides (ORCID: https://orcid.org/0000-0002-0878-0465)
- Shangtao Liu (ORCID: https://orcid.org/0000-0002-2724-9178)
- Hongxiang Zhang (ORCID: https://orcid.org/0000-0001-6794-0038)
- Ashley H. Choi (ORCID: https://orcid.org/0000-0002-2802-7163)
- Justin L. Engel (ORCID: https://orcid.org/0000-0002-2016-3494)
- Binzhang Shen (ORCID: https://orcid.org/0000-0002-6556-7791)
- Samuel R. Meier (ORCID: https://orcid.org/0000-0002-7026-2722)
- Brian J. McMillan (ORCID: https://orcid.org/0000-0002-3134-7774)
- Michele Meseonznik
Publication Details
- Published
- 2025-09-02
- DOI
- https://doi.org/10.1158/1535-7163.30028940
- Primary Topic
- PARP inhibition in cancer therapy
- Type
- article
- Field-Weighted Citation Impact
- 0.00