Hydroxylamine-O-Sulfonic Solid Acid as an Efficient Anti-Hemolytic Catalyst

Si@OSA-Dir and Si@OSA-Ref were produced by immobilization of immobilizing hydroxylamine-O-sulfonic acid (OSA) on rice husk ash in two different ways. The presence of Carbon, Sulfur, and Nitrogen in the silica was verified by elemental analysis. The specific surface area of both catalysts was 257 m2/g. The produced catalysts were shown to be stable at temperatures as high as 200 °C. The catalysts' cytotoxicity effects, antioxidant potency, and anti-hemolytic activity were evaluated in order to appraise evaluate their biological and medical applications. In contrast to the positive control (H2O2), which generated a high percentage of hemolysis, the results demonstrated that both catalysts exhibited low hemolytic activity at low concentrations of 100–150 μg/mL. Both catalysts exhibited strong antiradical activity and a generally high H2O2 scavenging activity percentage. The LDH assay demonstrated these catalysts safety and protective properties qualities. When erythrocytes treated with these two catalysts are exposed to hydrogen peroxide, the results are decreased to marginally reduced (554 U/L). These encouraging outcomes for both catalysts by enhancing antioxidant defenses, and scavenging harmful molecules shows promise in improving the efficacy to stabilize and stop the breakdown of red blood cells.