A Cupin Epoxidase Catalyzes Epoxycyclohexenone Formation in Fungal Meroterpenoid Biosynthesis
We identified the <i>par</i> biosynthetic gene cluster from <i>Paraphaeosphaeria</i> sp. C-XB-J-1, responsible for producing epoxycyclohexenone (ECH)-containing meroterpenoids, paraphaeones. In vitro reconstitution revealed the sequential action of the cupin-domain protein ParE and the SDR enzyme ParD in ECH formation. Molecular dynamics analysis and site-directed mutagenesis confirmed critical catalytic residues. This work provides the biochemical evidence for a fungal cupin epoxidase involved in ECH biosynthesis and expands the enzymology of fungal meroterpenoid.
Authors
- Yukai Suo (ORCID: https://orcid.org/0000-0001-8973-2109)
- Ping Zhao (ORCID: https://orcid.org/0000-0002-5618-0472)
- Wei‐Guang Wang (ORCID: https://orcid.org/0000-0003-3395-767X)
- Xuping Zhang (ORCID: https://orcid.org/0000-0001-9805-2316)
- Xiaojun Li (ORCID: https://orcid.org/0000-0001-8483-7438)
- Yuntao Jiang (ORCID: https://orcid.org/0000-0001-6320-6506)
- Wengang Xi
- Lian-Qiong Du (ORCID: https://orcid.org/0000-0001-9349-1459)
Institutions
- Minzu University of China (CN)
- Southwest Forestry University (CN)
- State Ethnic Affairs Commission (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1021/acs.orglett.5c03293
- Citations
- 2
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 1.39
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Yunnan Province
- Yunnan Provincial Department of Education Science Research Fund Project