Study of the cervical canal microbiome and microbiocenosis in reproductive-age women with squamous intraepithelial lesion

There are patients with HSIL on average older than patients with LSIL about 3 years. HPV 16 was found to be the most common virus type in patients with SIL. When determining of 16S rRNA genes of prokaryotic microorganisms in women of reproductive age with SIL by the new generation sequencing, it turned out that the cervical canal microbiome is inhabited by many atypical representatives (soil, aquatic and aerobacteria). In addition to the genera <i>Lactobacillus, Streptococcus, Staphylococcus, Gardnerella, Ureaplasma, Prevotella, Sneathia, Fusobacterium, Veillonella, Megasphaera, Dialister, Enterococcus, Escherichia/Shigella, Fannyhessea, Peptococcus, Peptostreptococcus, Finegoldia, Porphyromonas, Corynebacterium, Alloscardovia, Mageeibacillus, Haemophilus, Duncaniella, Ralstonia, Sphingomonas, Pedobacter, Methylobacterium, Ruminococcus, Sediminibacterium, Pseudomonas, Aerococcus, Acinetobacter, Campylobacter, Stenotrophomonas, Sphingobacterium, Phyllobacterium</i> and others may be included in the microbial composition of the cervical canal. Dysbiotic disorders were more common in patients with HSIL. <i>Streptococcus</i> spp. always accompanied aerobic vaginitis (AV), whereas bacterial vaginosis (BV) was associated with the genera <i>Gardnerella, Fannyhessea, Prevotella, Dialister, Sneathia, Anaerococcus, Megasphaera, Finegoldia, Peptoniphilus, Porphyromonas, Parvimonas</i> and <i>Eubacterium</i> spp. When comparing the two methods, the genera <i>Peptoniphilus, Methylobacterium, Ralstonia spp, Sphingomonas, Phyllobacterium, Parvimonas, Anaerococcus</i> and <i>Ruminococcus</i> may be included in the microbial biomass in a significant proportion. Eubacterium spp. did not occur in the 16S sequencing method. Some representatives are detected together with each other in the RT-qPCR method (Femoflor - 16), e.g., <i>Sneathia</i> spp. + <i>Leptotrichia</i> spp. + <i>Fusobacterium</i> spp.; <i>Megasphaera</i> spp. + <i>Veillonella</i> spp. + <i>Dialister</i> spp.; <i>Mobiluncus</i> spp. + <i>Corynebacterium</i> spp., however, <i>Corynebacterium</i> spp., <i>Veillonella</i> spp., <i>Mobiluncus</i> spp., <i>Fusobacterium</i> spp., <i>Leptotrichia</i> spp. and <i>Peptostreptococcus</i> spp. were practically absent (or in a low percentage) in the microbiome of women with SIL.