Decellularized porcine urinary bladder matrix as a dynamic biomaterial scaffold for myocardial cell adhesion

• A decellularized porcine bladder matrix (UBM) was developed as a biocompatible scaffold for myocardial tissue engineering. • A dynamic perfusion culture system was employed to enhance cardiomyocyte adhesion. • The UBM scaffold supports calcium ion release, indicating potential bioactivity for cardiac regeneration. • The platform supports the physiological behavior of myocardial cells and offers significant potential for future applications in cardiac tissue repair and drug screening systems. Porcine urinary bladder matrix (UBM) is a promising extracellular matrix-derived scaffold due to its excellent biocompatibility and structural integrity. Tissue engineering approaches for cardiac repair require scaffolds that promote myocardial cell adhesion and physiological function. This study investigates the potential of UBM as a dynamic culture scaffold for myocardial cell adhesion and activity. UBM scaffolds were prepared by decellularizing porcine bladder tissue to remove cellular components while preserving the extracellular matrix. Cardiomyocytes were seeded onto the UBM scaffold and cultured under dynamic conditions using a custom-designed perfusion system to simulate physiological flow. Calcium ion activity was monitored to assess the functionality of myocardial cells. The decellularized UBM scaffold demonstrated high compatibility and supported adequate adhesion of myocardial cells under dynamic culture conditions. Although cardiomyocytes remained viable and adhered well to the scaffold, the calcium assay used in this study measured extracellular calcium levels in the supernatant and does not reflect intracellular calcium flux or contractile activity. Nonetheless, the sustained release of calcium ions from the UBM scaffold suggests bioactivity that may contribute to supporting cardiac tissue development.