Reduction of apoptosis and preservation of stem-cell proliferation in intestinal transplantation allografts with normothermic machine perfusion
Background: Intestinal transplantation (IT) is the only lifesaving therapy for intestinal failure but is plagued by high rejection rates. Static cold storage (CS), the standard of allograft preservation, is known to induce damage. Normothermic machine perfusion (NMP) improves transplantation outcomes in other organs and may benefit IT. Preliminarily, NMP reduces apoptotic cells (CC3+), increases proliferative cells (Ki67+), and increases transplant recipient survival. We hypothesized that NMP upregulates anti-apoptotic, intestinal stem-cell proliferation, and wound healing pathways.
Authors
- Manuel Kleiner (ORCID: https://orcid.org/0000-0001-6904-0287)
- Elsa Ludwig
- Molly DuBois
- Katherine S. Garman (ORCID: https://orcid.org/0000-0001-7763-8465)
- Brittany Veerasammy (ORCID: https://orcid.org/0009-0006-7499-381X)
- Elizabeth Goya‐Jorge (ORCID: https://orcid.org/0000-0003-0515-989X)
- Cecilia R. Schaaf (ORCID: https://orcid.org/0000-0001-9900-8392)
- Nader Abraham (ORCID: https://orcid.org/0000-0001-9679-5642)
- Debra L. Sudan (ORCID: https://orcid.org/0000-0003-4315-4459)
- Ahmed O. Hassan (ORCID: https://orcid.org/0000-0002-9638-1260)
- D. L. ROSE (ORCID: https://orcid.org/0000-0003-1430-5692)
- Andrew S. Barbas (ORCID: https://orcid.org/0000-0003-3476-2313)
- Liara Gonzalez
- John Freund (ORCID: https://orcid.org/0009-0006-7530-4449)
- Amy Stewart (ORCID: https://orcid.org/0000-0003-1602-3461)
- Caroline McKinney-Aguirre
- J. Alfredo Blakeley‐Ruiz (ORCID: https://orcid.org/0000-0001-7638-5849)
- Célia Duarte Cruz (ORCID: https://orcid.org/0000-0001-8922-5713)
- Tobias Käser
- Juan Pesantez-Valdivieso
Institutions
- North Carolina State University (US)
- Duke University (US)
Publication Details
- Journal
- Intestinal failure.
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1016/j.intf.2025.100105
- Primary Topic
- Organ Transplantation Techniques and Outcomes
- Type
- article
- Field-Weighted Citation Impact
- 0.00