DESIGN AND CHARACTERIZATION OF OPTIMIZED POLYHERBAL NANOENCAPSULATION: IN VITRO ANTIOXIDANT ACTIVITY TARGETING NRF2 AND GST PATHWAYS

Objective: Antioxidants are compounds that can stop oxidation, protecting cells from the harmful effects of free radicals produced by the body's metabolism and some external sources. Flavonoid and phenolic compounds are secondary metabolites of plants that have high antioxidant activity. The secondary metabolites found in Nigella sativa seeds contain terpenoid and phenolic compounds, while the leaves of Moringa oleifera and Centella asiatica plants contain flavonoids, alkaloids, tannins, saponins, and steroids. The problem is that the extracts from Moringa oleifera and Centella asiatica have large particle sizes and poor solubility, while Nigella sativa oil contains volatile and easily oxidizable chemicals. Therefore, it is very important to create a drug delivery system that can enhance solubility and reduce particle size, one of which is nanoencapsulation. The research the aim is to determine the optimum formula in the polyherbal (Moringa oleifera, Centella asiatica and Nigella sativa) nanoencapsulation preparation as a method of drug delivery and antioxidant activity targeting Nrf2 and GST pathways using PBMC. Methods: Nanoencapsulation of polyherbal was carried out using chitosan and sodium tripolyphosphate polymers as crosslinkers and then characterizes. Optimization formulation by design expert (factorial design). In vitro analysis as an antioxidant in the regulation of Nrf2 and GST using PBMC with the flow cytometry method. Results: The particle size, PDI and zeta potential from the optimum formula values were found 115.463±9.563 nm; 0.49±0.050, and-29.737±1.665mV. The Nrf2 and GST enhancement activity, it is evident that the nanopolyherbal preparations with concentrations of 3% and 1% have higher Nrf2 expression values compared to the control. Conclusion: Nanoencapsulation of polyherbal (Moringa oleifera, Centella asiatica and Nigella sativa) have good antioxidant effect by Nrf2 and GST regulation, so it can be used as drug delivery system method.