Proteomic profiles in the aqueous following anti-vegf therapy in treatment naïve neovascular age-related macular degeneration
In this exploratory study, 56 proteins were identified that could potentially be linked with NVAMD. In interference studies free aqueous VEGF levels declined while total VEGF levels increased following anti-VEGF treatment. No large off-target effects on the proteome were observed with treatment. We illustrate how the protein interactome can mask or potentially unmask binding epitopes leading to signal changes not necessarily related to the absolute protein level.
Authors
- Nebojša Janjić (ORCID: https://orcid.org/0000-0001-6811-167X)
- Shashi Kumar Gupta (ORCID: https://orcid.org/0000-0003-4898-9877)
- Alan G. Palestine (ORCID: https://orcid.org/0000-0001-6361-6767)
- Naresh Mandava (ORCID: https://orcid.org/0000-0003-3251-6469)
- Matthew J. Westacott (ORCID: https://orcid.org/0000-0001-6049-2420)
- Talisa E. de Carlo (ORCID: https://orcid.org/0000-0002-3264-8982)
- Jennifer L. Patnaik (ORCID: https://orcid.org/0000-0002-0375-0187)
- Brandie D. Wagner (ORCID: https://orcid.org/0000-0002-2745-0103)
- Anne M. Lynch (ORCID: https://orcid.org/0000-0001-6508-8136)
- Daniel W. Drolet (ORCID: https://orcid.org/0000-0002-1877-0092)
- Kinsey M. Trinder
Institutions
- Colorado School of Public Health (US)
- The Medical Center of Aurora (US)
- SomaLogic (United States) (US)
- University of Colorado Denver (US)
Publication Details
- Journal
- Clinical Proteomics
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1186/s12014-025-09555-3
- Primary Topic
- Retinal Diseases and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Research to Prevent Blindness
- Georgia Clinical and Translational Science Alliance
- National Institutes of Health
- National Center for Advancing Translational Sciences