Advantages of Femtosecond Laser Microdrilling PDMS Membranes over Conventional Methods for Organ-on-a-Chip

Organ-on-a-chip (OoC) technology aims to replicate the functions of human organs and tissues. This study evaluates femtosecond laser micromachining (FLM) for producing PDMS membranes with controlled porosity as an alternative approach to conventional microfabrication for OoCs. Membranes of varying thicknesses were microdrilled, and the influence of laser parameters on microhole geometry was assessed, showing that pulse energy strongly affected hole diameter, whereas exposure time had a lesser impact. The heat-affected zone (HAZ) and taper angle, key indicators of microhole geometric quality, were also analyzed and found to be strongly dependent on membrane thickness. Prediction models were developed to guide parameter selection for future laser-based ablation processes. A numerical model that predicts plasma shielding effects provided further insight into the physics of PDMS laser ablation, revealing that higher pulse energies led to a marked increase in crater diameter. The fabricated membranes were integrated into an OoC device, onto which human mesenchymal stem cells were seeded. The results demonstrated strong cell adhesion, the rapid formation of a homogeneous monolayer, and no evidence of cytotoxicity. These findings confirm that FLM is a versatile and flexible technique for microdrilling PDMS membranes, enabling their effective integration into OoC.