Scientific Evaluation of Siddha Formulation Sirupanchamoola Kiyazham: Antimicrobial Activity, Molecular Docking and Toxicological Safety Assesments

The Siddha system of medicine, deeply rooted in Tamil Nadu's traditional practices, emphasizes holistic health through herbal and mineral formulations. Combining antibacterial activity, molecular docking mechanisms, and toxicological safety assessments, this work offers a thorough scientific assessment of Sirupanchamoola Kiyazham (SPK), a Siddha preparation. Safety and efficacy of SPK were evaluated using advanced methods including toxicity studies in Wistar albino rats, High-Performance Thin-Layer Chromatography (HPTLC), and Thin-Layer Chromatography (TLC). The antimicrobial study verified sterility of SPK by not revealing any microbial contamination. In Docking study, the binding of solanine, quercetin, luteolin, and lupeol to Cyclooxygenase-2 (COX-II) and Prostaglandin H synthases highlights the potential of Sirupanchamoola Kiyazham as an analgesic and anti-arthritic agent, HPTLC fingerprinting identified important phytocompounds. Acute and 28-day repeated-dose oral toxicity tests as well as other toxicological evaluations found no appreciable side effects, therefore verifying the safety of SPK within clinical dosage ranges. The results confirm the therapeutic potential of SPK and support its safe integration into contemporary medicine. This research bridges the gap between traditional knowledge and scientific validation, paving the way for future clinical trials to establish its efficacy in managing pain related conditions.