Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli
The N191/192C split Dre protein and the intein-fused N60/61C split Dre protein can effectively mediate recombination of the integrated rox sites without the need for external signals such as light or chemical compounds. Split Dre and Cre proteins can be used together in the same cell without interfering with each other. These findings introduce new tools and strategies for gene editing and the generation of transgenic animals.
Authors
- Chichu Xie (ORCID: https://orcid.org/0000-0002-5141-8875)
- Yu Liu (ORCID: https://orcid.org/0000-0002-3858-7578)
- Wentao Yu (ORCID: https://orcid.org/0000-0002-2348-5050)
- Jie Gan (ORCID: https://orcid.org/0009-0001-1682-0068)
- Guangying Qi
- Song Guo Zheng (ORCID: https://orcid.org/0000-0002-5611-4774)
- Yuanhao Zhang (ORCID: https://orcid.org/0000-0003-3448-8924)
- Dan Yu
- Ruirui Li (ORCID: https://orcid.org/0009-0008-7662-1213)
Institutions
- Sun Yat-sen University (CN)
- Guilin Medical University (CN)
- Songjiang District Central Hospital (CN)
- Third Affiliated Hospital of Sun Yat-sen University (CN)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1186/s13036-025-00551-7
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China