Streptomyces mayonensis sp. nov., isolated from the volcanic soils of Mt. Mayon, Philippines

A novel actinobacterium, designated as strain A1-08^T, was isolated from the volcanic soils of Mt. Mayon, Philippines. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain A1-08^T belonged to the genus <i>Streptomyces</i> with sequence similarity with <i>Streptomyces djakartensis</i> NBRC 15409^T (99.02%), <i>Streptomyces tuirus</i> NBRC 15617^T (98.88%) and <i>Streptomyces mutabilis</i> NBRC 12800^T (98.81%). Polyphasic characterization demonstrated distinct morphological, cultural, physiological and biochemical features with unique lipid and fatty acid profiles and respiratory quinones. The major phospholipids of strain A1-08^T are diphosphatidylglycerol, phosphatidylethanolamine, glycophospholipid with mannose and/or galactose and unidentified lipids such as aminolipid and phospholipid. The major cellular fatty acids were anteiso-C_{15 : 0}, C_{16 : 0}, anteiso-C_{17 : 0}, iso-C_{16 : 0}, iso-C_{15 : 0} and C_{16 : 1} <i>ω</i>7c. The respiratory quinones of the strain include MK-9 (H6), MK-9 (H8), MK-10 (H6) and MK-10 (H8). Multilocus analysis using the concatenated sequences of <i>atpD</i>, <i>gyrB</i>, <i>recA</i>, <i>rpoB</i> and <i>trpB</i> showed that the strain formed a distinct branch in the genus <i>Streptomyces.</i> Potential genes for environmental stress response and adaptation were identified in the genome of strain A1-08^T and its closest relative strains. Whole-genome sequencing confirmed A1-08^T as a novel species within <i>Streptomyces</i> with 57.80% digital DNA-DNA hybridization and 93.03% average nt identity value with the closest type strain <i>Streptomyces olivaceus</i> NRRL B-3009^T. The name <i>Streptomyces mayonensis</i> sp. nov., derived from the Mayon Volcano in the Philippines, is hereby proposed (type strain A1-08^T =JCM 36345, =DSM 118395).