The adaptor protein SKT interacts with PSD-95 and SHANK3 and affects synaptic functions
Postsynaptic density (PSD) is a tightly interconnected protein network ensuring synaptic function through the interaction of neurotransmitter receptors, structural adaptor proteins, and signaling molecules. Disruption of PSD may cause neurological diseases, including autism spectrum disorders and cognitive impairment. We demonstrate that the SKT adaptor distinctly localizes within dendritic spines as an integral component of the synaptic network, binding PSD-95 and SHANK3. SKT-knockout (KO) mice show significant abnormalities in dendritic spine density and morphology, consistent with RhoA and Rac1 GTPase dysregulated activity. KO-derived neuronal cultures display delayed neuronal synchronization and maturation associated with glutamatergic pre- and postsynaptic impairment. Behavioral tests on KO mice reveal increased self-grooming activity and impaired motor coordination, with altered cognitive and executive functions compared to wild-type mice. Overall, SKT emerges as a key contributor to the structural and functional PSD organization, regulating synaptic function through its interactions with PSD components.
Authors
- Paolo Mele (ORCID: https://orcid.org/0000-0002-5685-6663)
- Antonia Gurgone (ORCID: https://orcid.org/0000-0002-6128-6466)
- Carola Eva (ORCID: https://orcid.org/0000-0002-9638-7240)
- Vincenzo Salemme (ORCID: https://orcid.org/0000-0001-9431-9693)
- Annalisa Alfieri
- Giorgia Centonze (ORCID: https://orcid.org/0000-0003-3923-8350)
- Marta Gai (ORCID: https://orcid.org/0000-0002-9819-1743)
- Andrea Becchetti (ORCID: https://orcid.org/0000-0002-9297-0527)
- Maurizio Giustetto (ORCID: https://orcid.org/0000-0003-1323-4060)
- Olga Teresa Bianciotto
- Alessandra Raspanti (ORCID: https://orcid.org/0000-0002-2394-8465)
- Valentina Carabelli (ORCID: https://orcid.org/0000-0002-8718-1208)
- Paola Defilippi (ORCID: https://orcid.org/0000-0001-6427-4906)
- Enis Hidişoğlu (ORCID: https://orcid.org/0000-0002-1729-1209)
- Daniela Gavello (ORCID: https://orcid.org/0000-0001-5882-4836)
- Andrea Marcantoni (ORCID: https://orcid.org/0000-0001-5825-7827)
- Mario De Gregorio
- Giuseppe Chiantia (ORCID: https://orcid.org/0000-0001-7971-4994)
- A Lodi
- Emilio Carbone (ORCID: https://orcid.org/0000-0003-2239-6280)
- Ilaria Bertocchi (ORCID: https://orcid.org/0000-0002-2880-716X)
- Alessandro Morellato
- Tiziana Cravero
- Costanza Angelini (ORCID: https://orcid.org/0000-0002-9662-4066)
- Dora Natalini (ORCID: https://orcid.org/0000-0001-8247-2893)
- Francesca Nigrelli
- Giacomo D’Attanasio
- Rebecca Oddone (ORCID: https://orcid.org/0009-0000-0926-887X)
- Emilia Turco
- Federico Torelli
- Vittorio Belmonte
- Beatrice Bersia
Institutions
- Izmir University (TR)
- University of Freiburg (DE)
- University Medical Center Freiburg (DE)
- Neuroscience Institute (IT)
- University of Turin (IT)
- University of Milano-Bicocca (IT)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1016/j.celrep.2025.116206
- Citations
- 1
- Primary Topic
- Genetics and Neurodevelopmental Disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.82
Funders
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Associazione Italiana per la Ricerca sul Cancro