Generation of a PHF19 knockout human embryonic stem cell line by CRISPR/Cas9 system
PHD finger protein 19 (PHF19) is a polycomb protein that promoted cardiac hypertrophy via epigenetic targeting SIRT2. To determine the role of PHF19 in myocardial hypertrophy, we established a large fragment knockout model of PHF19 gene in human embryonic stem cells (hESCs-H7) using the CRISPR/Cas9 system based on a vector. This PHF19-KO cell line has a normal karyotype, classical human pluripotent stem cell morphology, strong pluripotency, and significantly reduced PHF19 gene expression, which will become a useful tool for further in-depth research on the pathogenesis of PHF19 gene deficiency induced myocardial hypertrophy.
Authors
- Xiaoling Guo (ORCID: https://orcid.org/0000-0003-3153-6249)
- Yuting Guan (ORCID: https://orcid.org/0000-0003-1866-270X)
- Feng Xing (ORCID: https://orcid.org/0000-0003-0564-2187)
- Yijia Wang (ORCID: https://orcid.org/0000-0002-4026-9245)
- Pingping Tan (ORCID: https://orcid.org/0009-0006-5027-7747)
- Yuqing Ran
- Jinghua Ruan
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Wenzhou Medical University (CN)
- Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1016/j.scr.2025.103824
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00