Refining Biodegradability Assessments of Polymers Through Microbial Biomolecule Quantification

The accumulation of plastic waste has intensified the pursuit of biodegradable alternatives, yet standard methods such as CO₂ evolution, oxygen demand, and mass loss fail to fully capture microbial physiological responses during degradation. This study introduces a biochemical assay-based approach to quantify proteins, lipids, and carbohydrates in soil as indicators of microbial activity during polymer biodegradation. For microcrystalline cellulose (MCC), proteins, lipids, and carbohydrates increased by 2.09-, 6.47-, and 11.22-fold, respectively (all <i>p</i>-values < 0.001), closely aligning with CO₂ evolution trends. Non-biodegradable poly(vinyl chloride) (PVC) exhibited no significant changes. Synthesized poly(butylene glutarate) (PBG) also showed significant biomolecule accumulation (up to 2.70-fold) alongside CO₂ production. Biomolecule quantification complements CO₂-based methods by revealing microbial proliferation and metabolic activity that persist beyond the mineralization plateau, offering a more comprehensive assessment of biodegradability.