Quantitative Fluorescence Assay Using Indocyanine Green in Early Diagnosis of Acute Small Intestinal Ischemia: An Experimental Study

Intraoperative assessment of tissue viability of hollow organs of the gastrointestinal tract during surgical interventions for acute mesenteric ischemia and a number of other surgical operations remains an unsolved problem. Existing methods for determining tissue viability limits in conditions of acute circulatory failure are characterized by a low sensitivity, which determines the choice of surgical treatment tactics for the patient. The aim of the work was to assess the effectiveness of the intraoperative fluorescence visualization technique for ischemic changes in the intestinal wall using a new device “MMC Scope ICG”, which allows using the V / Q-ICG method of research for quantitative angiography with indocyanine green (ICG), performed in the near infrared range. Wistar rats were divided into four groups: 1) 15-minute, 2) 30-minute, 3) 45-minute and 4) 60-minute intestinal ischemia, which after 30 minutes of reperfusion underwent recordings of curves of changes in time of ICG fluorescence brightness in zones with ischemic and reperfusion damage of intestinal tissue in comparison with control areas of healthy intestine. The analysis of relationship between changes in the indicated dynamic curves of indocyanine green fluorescence intensity and typical morphological changes in rat intestinal samples shows direct (positive) relationship between the dynamics of the increase in indocyanine green fluorescence intensity and the degree of morphological changes in the damaged area of the intestine