Retinoid acid-related orphan receptor alpha serum level and gene polymorphism in psoriatic patient

Background Retinoid acid-related orphan receptor alpha (RORA) has been implicated in various inflammatory and autoimmune conditions, including psoriasis. Objectives To investigate the association between RORA gene polymorphisms (rs11639084 and rs4774388) and susceptibility to psoriasis, as well as to evaluate serum RORA protein levels in psoriatic patients compared to healthy controls. Patients and methods A case–control study was conducted involving 100 participants, including 60 psoriatic patients and 40 age-matched and sex-matched healthy controls. Genotyping of RORA single nucleotide polymorphisms (rs11639084 and rs4774388) was performed using the tetra-primer amplification refractory mutation system-PCR. Serum RORA protein levels were measured by enzyme-linked immunosorbent assay. Results Significant associations were found between RORA polymorphisms and psoriasis. For rs11639084, the TC and CC genotypes were more frequent in patients than controls ( P =0.005 and 0.012, respectively), with a dominant model odds ratio of 4.86 ( P =0.001). For rs4774388, the CT and TT genotypes were also more common in patients ( P =0.017 and 0.009, respectively), with a dominant model odds ratio of 4.46 ( P =0.003). Serum RORA levels were higher in psoriatic patients (median: 23.82 ng/ml) compared to controls (17.67 ng/ml, P <0.001) and correlated with disease severity (psoriasis areas and severity index: r =0.973, P <0.001; nail psoriasis severity index: r =0.750, P <0.001). Conclusions The study demonstrates a significant association between RORA gene polymorphisms and psoriasis, with elevated serum RORA protein levels correlating with disease severity.