Streamlined Protocol for sgRNA validation (post-design), Popsuj et al. 2024 v1

Compiled here is the wet lab protocol for CRISPR/Cas9 sgRNA validation by NGS, assuming already-designed sgRNAs and primers.

Authors

Publication Details

Published
2025-08-29
DOI
https://doi.org/10.17504/protocols.io.14egnr97yl5d/v1
Citations
1
Primary Topic
Advanced biosensing and bioanalysis techniques
Type
article
Field-Weighted Citation Impact
0.81
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article

Streamlined Protocol for sgRNA validation (post-design), Popsuj et al. 2024 v1

Popsuj et al.
1 citations
Advanced biosensing and bioanalysis techniques
0.81
article

Streamlined Protocol for sgRNA validation (post-design), Popsuj et al. 2024 v1

Popsuj et al.
article en
1 citations

Abstract

Compiled here is the wet lab protocol for CRISPR/Cas9 sgRNA validation by NGS, assuming already-designed sgRNAs and primers.

Openalex Percentile: Top 24%
Advanced biosensing and bioanalysis techniques
0.81
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Streamlined Protocol for sgRNA validation (post-design), Popsuj et al. 2024 v1 — Popsuj et al. · (2025) | TGRS Research Map | TGRS