Rapid and accurate detection method for bluetongue virus based on CRISPR-Cas13a combined with RT-ERA
This work represents the first CRISPR-Cas13a-based platform for pan-serotype BTV detection, combining portability, cost-efficiency, and detective accuracy suitable for point-of-care deployments. By bridging the gap between high laboratory sensitivity and practical field applicability, this system enables real-time BTV surveillance and facilitates timely outbreak containment in resource-constrained agricultural and veterinary settings.
Authors
- Yi Shao (ORCID: https://orcid.org/0000-0002-3237-8310)
- Haibo Yu (ORCID: https://orcid.org/0000-0003-1506-2346)
- Yinglin Qi (ORCID: https://orcid.org/0009-0008-9773-7864)
- Changyou Xia (ORCID: https://orcid.org/0000-0002-2701-1143)
- Caixia Gao (ORCID: https://orcid.org/0000-0003-3169-8248)
- Dong Zhou (ORCID: https://orcid.org/0000-0002-6565-0421)
Institutions
- Harbin Veterinary Research Institute (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-09-01
- DOI
- https://doi.org/10.3389/fcimb.2025.1621012
- Primary Topic
- Vector-Borne Animal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Chinese Academy of Agricultural Sciences