Investigating alveolar macrophages in an human ex vivo precision‐cut lung slice model of SARS‐CoV‐2 infection using Raman spectroscopy—A case study
Raman spectroscopy can characterize the cellular immune response and phenotype plasticity of AMs to infection with SARS-CoV-2 within a PCLS model in a label-free and non-invasive manner. The ability to distinguish cells from infected PCLSs from cells of the uninfected control PCLS based on intracellular biochemical changes highlights the potential of Raman spectroscopy as a powerful diagnostic tool in immunology and clinical diagnostics.
Authors
- Antje Häder (ORCID: https://orcid.org/0000-0001-8496-1754)
- Stefanie Deinhardt‐Emmer (ORCID: https://orcid.org/0000-0003-4495-4052)
- Rustam R. Guliev (ORCID: https://orcid.org/0000-0001-8910-4137)
- Astrid Tannert (ORCID: https://orcid.org/0000-0003-4115-2564)
- Simone Eiserloh
- Ute Neugebauer
- Franziska Hornung (ORCID: https://orcid.org/0000-0002-9942-5572)
- Max Naumann (ORCID: https://orcid.org/0000-0002-9067-1232)
- T. Sandhaus
Institutions
- Leibniz Institute of Photonic Technology (DE)
- Jena University Hospital (DE)
- Friedrich Schiller University Jena (DE)
Publication Details
- Journal
- Clinical and Translational Medicine
- Published
- 2025-08-31
- DOI
- https://doi.org/10.1002/ctm2.70453
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Freistaat Thüringen
- Bundesministerium für Bildung und Forschung
- Leibniz-Gemeinschaft
- European Regional Development Fund
- Center for Sepsis Control and Care