Occurrence and Genetic characterization of Fasciola spp. Infections

Fasciolosis, caused by Fasciola species, is a significant parasitic disease affecting sheep, particularly in tropical and subtropical regions.This study aimed to assess the occurrence and molecular identification of Fasciola species in sheep from Patna, Bihar.A total of 445 sheep from various age groups, sexes, and body conditions were screened for fasciolosis through coprological examination.The overall occurrence rate was 25.16%, with a higher prevalence in females, sheep aged 1-3 years, and those with poor body condition.Molecular analysis of Fasciola samples revealed Fasciola gigantica as the only species present, identified through PCR amplification of the ITS-1 rRNA gene.Phylogenetic analysis showed high genetic similarity between Patna isolates and those from regions like Vietnam, Thailand, and Egypt.These findings highlight the importance of early detection and control measures for fasciolosis in sheep farming in Patna and can serve as a foundation for future control strategies in the region and state.