Label-free detection of DNA Amplification using Plasmonic Crystal

The polymerase chain reaction (PCR) is widely used for the detection of specific sequences of DNAs. PCR can easily amplify the target DNAs; however, PCR requires a labeling procedure for the detection of amplified DNAs. In this study, label-free detection of amplified DNAs based on localized surface plasmon resonance (LSPR) using gold layer deposited plasmonic crystal (PC) was developed. Label-free detection of amplified DNAs by PCR was carried out on the PC, and the LSPR optical characteristics change was monitored. As a result, the possibility of label-free detection of DNAs using PC was successfully suggested.