Time-lapse Imaging of Actinomycete <i>Amycolatopsis thailandensis</i> with a Microwell

High-throughput culture of actinomycetes using commercially available micro-droplets is difficult because the elongated mycelium penetrates other microdroplets and destroys them. In this study, we developed a microwell with independent micro-spaces and succeeded in culturing actinomycetes while maintaining them in each micro-space. We also assessed their growth behavior by continuously analyzing the autofluorescence of the same cells.