Detection the Expression of two Genes ZmWRKY86 and PMP3 responsible for tolerance to salt stress in maize

The current study measured the expression of certain genes responsible for the tolerance of the maize (Zea mays L.) to salt stress when it was grown in soil containing (0.0,25, 50,75,100 mmol) sodium chloride (NaCl) for 30 days. The results indicated that the germination percentage decreased with the increased salt concentration. Isolated ribonucleic acid (RNA) from young leaves of maize seedlings was used as a template for manufacturing a piece of complementary nucleic acid (cDNA), gene expression was measured by Reverse Transcription Polymerase Chain Reaction (RT-PCR) for both PMP3 and ZmWRKY86 genes responsible for salinity stress tolerance. The expression level of the PMP3 gene increased with increasing salt concentration and reached 1.38 and 1 .86 at concentrations of 50 and 75 mmol, respectively, but decreased with increasing salt concentration to 100 mmol by 0.34%. The expression of the second gene ZmWRKY86 was increased with increasing salt concentration and reached a maximum level of 3.18 at 100 mmol of NaCl.