Identification of Protein Types and Molecular Weight of Mangrove Snail (Telescopium Telescopium) Isolate at Different pH

Mangrove snail (Telescopium Telescopium) is a gastropods species with low economic value serving as an alternative source of animal protein in the form of isolate. Therefore, this research aimed to evaluate the effect of different pH values in the yield, proximate composition, soluble protein content, and molecular weight of mangrove snail isolate. An experimental method was used with completely randomized design (CRD) consisting of three pH treatment levels (4.5; 5.5; 6.5) and three replications. Furthermore, data were subjected to descriptive statistics and analysis of variance (ANOVA). The stages included sample preparation, grinding of mangrove snail meat, and production of protein isolates at different pH levels. The results showed that mangrove snail flour, moisture content, ash content, and protein content had a yield of 25.52%, 5.27%, 4.58%, and 42,88%, respectively. The highest protein isolate yield (5,06%) and soluble protein contenct (1.90 mg/mL) were obtained at pH 4.5. molecular weight profiles varied by 4-191, 4-44, and 4-54 kDa at pH 6.5, 5.5, and 4.5, respectively. Protein identification reported the presence of actin, α-tropomyosin, myosin light chain, lysozyme, and peptides, suggesting potential applications in health food products such as protein supplements.