Physiological, biochemical and enzymatic quality parameters of primed seed of rapeseed-mustard genotypes

High-quality seed of improved genotypes is a critical key to well attract the consumers. Therefore, a two-year laboratory experiment was carried out at Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India in 2018 and 2019 in a triplicated factorial completely randomized design to determine the quality parameters of the newly produced seeds from plants of six rapeseed-mustard genotypes (Anushka and Sanchita (rapeseed), TBM-204 and TBM-143 (yellow seed coated mustard), Kranti and Pusa Bold (black seed coated mustard)) grown under five seed priming (KH<sub>2</sub>PO<sub>4</sub> (0.15 mol), KNO<sub>3</sub> (0.1 mol), polyethylene glycol (PEG) 6000 (-0.3 MPa), distilled water, and dry seed). Pusa Bold was recognized to have greater germination percentage under application of KH<sub>2</sub>PO<sub>4</sub> (86.32%). Further, KH<sub>2</sub>PO<sub>4</sub> registered high root length (TBM-204) and seedlings fresh (TBM-204) and dry weights (Pusa Bold). Pusa Bold under application of KH<sub>2</sub>PO<sub>4</sub> also expressed the highest soluble protein (23.05%), while Anushka had maximum oil content under seed treatment with KH<sub>2</sub>PO<sub>4</sub> (46.30%) as well as lowest electrical conductivity (0.91 µS m<sup>- 1</sup> g<sup>- 1</sup>). Additionally, KH<sub>2</sub>PO<sub>4</sub> had more impact on Pusa Bold regarding α- amylase activity, while TBM-143 treated with KH<sub>2</sub>PO<sub>4</sub> recorded highest peroxidase activity. So, using KH<sub>2</sub>PO<sub>4</sub> to prime Pusa Bold seeds can be an effective way to enhance germination, seedling vigour, and biochemical characteristics, which will increase oilseed crop productivity and quality. By encouraging sustainable agriculture and raising crop production, this strategy supports the UN sustainable development goal (SDG) 2 (zero hunger). Additionally, it meets SDG 12 (responsible consumption and production) through sustainable resource management and decreased environmental effect by optimizing input consumption and improving seed performance without genetic modification or excessive chemical use.

Authors

Institutions

Publication Details

Journal
Scientific Reports
Published
2025-08-30
DOI
https://doi.org/10.1038/s41598-025-09325-z
Primary Topic
Phytase and its Applications
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Physiological, biochemical and enzymatic quality parameters of primed seed of rapeseed-mustard genotypes

Saikat Biswas, Rupa Das, Amitava Dutta
Scientific Reports
Phytase and its Applications
article

Physiological, biochemical and enzymatic quality parameters of primed seed of rapeseed-mustard genotypes

Saikat Biswas, Rupa Das, Amitava Dutta
article en

Abstract

High-quality seed of improved genotypes is a critical key to well attract the consumers. Therefore, a two-year laboratory experiment was carried out at Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India in 2018 and 2019 in a triplicated factorial completely randomized design to determine the quality parameters of the newly produced seeds from plants of six rapeseed-mustard genotypes (Anushka and Sanchita (rapeseed), TBM-204 and TBM-143 (yellow seed coated mustard), Kranti and Pusa Bold (black seed coated mustard)) grown under five seed priming (KH<sub>2</sub>PO<sub>4</sub> (0.15 mol), KNO<sub>3</sub> (0.1 mol), polyethylene glycol (PEG) 6000 (-0.3 MPa), distilled water, and dry seed). Pusa Bold was recognized to have greater germination percentage under application of KH<sub>2</sub>PO<sub>4</sub> (86.32%). Further, KH<sub>2</sub>PO<sub>4</sub> registered high root length (TBM-204) and seedlings fresh (TBM-204) and dry weights (Pusa Bold). Pusa Bold under application of KH<sub>2</sub>PO<sub>4</sub> also expressed the highest soluble protein (23.05%), while Anushka had maximum oil content under seed treatment with KH<sub>2</sub>PO<sub>4</sub> (46.30%) as well as lowest electrical conductivity (0.91 µS m<sup>- 1</sup> g<sup>- 1</sup>). Additionally, KH<sub>2</sub>PO<sub>4</sub> had more impact on Pusa Bold regarding α- amylase activity, while TBM-143 treated with KH<sub>2</sub>PO<sub>4</sub> recorded highest peroxidase activity. So, using KH<sub>2</sub>PO<sub>4</sub> to prime Pusa Bold seeds can be an effective way to enhance germination, seedling vigour, and biochemical characteristics, which will increase oilseed crop productivity and quality. By encouraging sustainable agriculture and raising crop production, this strategy supports the UN sustainable development goal (SDG) 2 (zero hunger). Additionally, it meets SDG 12 (responsible consumption and production) through sustainable resource management and decreased environmental effect by optimizing input consumption and improving seed performance without genetic modification or excessive chemical use.

Scientific ReportsVol. 15(1)
Bidhan Chandra Krishi Viswavidyalaya (IN), Ramakrishna Mission Vidyamandira (IN), Ramakrishna Mission Vivekananda Educational and Research Institute (IN), Usha Martin (India) (IN)
Openalex Percentile: Top 83%
Phytase and its Applications
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.