Attenuating effect of Lacticaseibacillus rhamnosus SDSP202406 on antibiotic-induced neurotransmitter imbalance in mice

Probiotics are active microorganisms beneficial to health when given in sufficient quantities. This study explored the protective effect of Lacticaseibacillus rhamnosus SDSP202406 against antibiotic-induced locomotor disorders using a processed antibiotic mixture to construct locomotor disorders in mice. The results showed that Lacticaseibacillus rhamnosus SDSP202406 significantly increased endurance performance metrics in both swimming and running behavioral paradigms ( P < 0.01), significantly decreased the expression of inflammatory factors, increased the superoxide dismutase and decreased the malondialdehyde in the cerebrum. Moreover, it enhanced the expression of brain-derived neurotrophic factor metabolic pathways in the cerebrum. Additionally, correlation analysis revealed relationship between gut microbiota and cerebrum neuroinflammation. It can be hypothesized that oral Lacticaseibacillus rhamnosus SDSP202406 caused the reprogramming of gut microbiota metabolism, promotes neurological homeostasis. The results demonstrated a modulating effect of Lacticaseibacillus rhamnosus on antibiotic-induced locomotor dysfunction in mice, providing a theoretical basis for the development of probiotic preparations to enhance lo-comotor function. • Lacticaseibacillus rhamnosus gut-brain axis mechanism alleviates movement disorders. • Multidimensionally confirms neuroprotective, anti-inflammatory, antioxidant effects. • Potential probiotic intervention strategies to combat antibiotic-associated neurobehavioral side-effects.