An engineered human hepatitis A virus capable of rapid proliferation in vitro and causing hepatitis in mice
Hepatitis A virus (HAV) is an ongoing public health concern, yet experimental tools to study recombinant HAV mutants remain limited. We developed a genetically tractable recombinant virus (HAV-2m) that replicates efficiently in cell culture, induces acute hepatitis in mice, and revealed a novel virulence determinant in the viral 2C protein. This platform enables detailed dissection of HAV pathogenesis and the evaluation of antiviral countermeasures.
Authors
- Tian-Shu Cao
- Quan Chen (ORCID: https://orcid.org/0000-0001-7539-8728)
- Peiyu Jiang
- Cheng‐Feng Qin (ORCID: https://orcid.org/0000-0002-0632-2807)
- Jian Li (ORCID: https://orcid.org/0000-0001-7953-8230)
- Min Gao (ORCID: https://orcid.org/0000-0001-5284-4828)
- Xiuli Yan (ORCID: https://orcid.org/0000-0003-0015-2138)
- Hui Zhao (ORCID: https://orcid.org/0000-0003-0576-1012)
- Xiaoyan Wu (ORCID: https://orcid.org/0000-0003-1716-6245)
- Ruo-Tong Ruan
Institutions
- Anhui Medical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Academy of Military Medical Sciences (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- JHEP Reports
- Published
- 2025-08-30
- DOI
- https://doi.org/10.1016/j.jhepr.2025.101582
- Citations
- 1
- Primary Topic
- Hepatitis Viruses Studies and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.32
Funders
- Chinese Academy of Medical Sciences